RESEARCH ARTICLES

Volume 8 |Issue 4| July-August 2020                                 First published: 30 Augest 2020

Cell immobilization by Agar-Agar & Agarose for protease production by thermotolerant Bacillus licheniformis JX849145

1Bhusare DU,2Wakte PS,3Kulkarni SS and 3Bhate MA

1Department of Microbiology, Digambarrao Bindu ACS College, Bhokar, (MS) India

2Department of Microbiology, Dnyanopasak College, Parbhani (MS) India

3Department of Microbiology, Shri Shivaji College Parbhani. 431401 (MS) India.

Abstract

In the present investigation, thermotolerant Bacillus licheniformis JX849145 was immobilized with gel matrices such as Agar-agar and Agarose. Agarose is a superficial form of agar-agar which is commonly used in to maintain the porosity for mobilization of genetic material. In the present study various concentration were used of Agar and agarose such as 2, 4, 6, 8, 10% and 1, 2, 3% respectively. At 4% of agar 0.78 U/mg/min highest enzyme activity was noted at 24 hrs of incubation period where as 3% of agarose the 0.69 U/mg/min highest activities was found at 66 hrs of incubation period. Comparatively Agar-agar has taken lower time for enzyme production and agarose has taken more time and less production is occurred as compared to agar-agar gel matrix. Hence agar-agar is found more efficient than the agarose for protease production by cell immobilization.

Keywords:Bacillus licheniformis, Protease, Immobilization, Gel Matrixes,

Editor: Dr.Arvind Chavhan

Cite this article as:
Bhusare DU, Wakte PS, Kulkarni SS and Bhate MA. Cell immobilization by Agar-Agar & Agarose for protease production by thermotolerant Bacillus licheniformis JX849145, Int. Res. Journal of Science & Engineering, 2020, Volume 8(4): 131-136.

Copyright:

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