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RESEARCH ARTICLES

Volume 9 |Issue 1| January-February 2021                        First published: 28 February 2021

Study on biosurfactant production by beneficial lactic acid bacteria isolated from traditionally fermented Indian foods

Ghume Vaishnavi, Shingade Shilpa , Walhe Rajan*

Department of Microbiology, Abasaheb Garware College, Pune,

*Corresponding Author: Walhe Rajan, Associate Professor and Head, Department of microbiology, MES’ Abasaheb Garware College, Pune (India). E-mail: rawalhe@rediffmail.com

Abstract

Biosurfactants are also produced by various microorganisms which are isolated from traditionally fermented Indian foods. The properties of biosurfactants are same as those of chemical surfactants but biodegradable, less toxic and compatible with the human skin. There are various biosurfactants known such as glycolipids, rhamnolipids, lipopeptides, phospholipids, fatty acids and polymeric biosurfactant, etc. LAB are believed to have positive health effects that are present in fermented foods with probiotic properties. In the present study, we have isolated a total of 56 isolates of LAB from different traditionally fermented Indian foods. The LAB was characterized on the basis of primary tests for probiotics. Out of 56 isolates, 29 isolates were found to be non-hemolytic of that, 20 isolates were able to tolerate acid at pH 2.0, for 120 minutes also able to tolerate bile at 0.3% concentration, 17 isolates showed BSH activity. Selected isolates were screened for biosurfactant activity by using drop collapse assay and oil spreading assay. Three isolates, out of 17 isolates shown primary tests for biosurfactant activity also, the thin layer chromatography (TLC) method was performed.

Keywords:Fermented foods, Lactic acid bacteria, BSH activity, Biosurfactants, Probiotics

Editor: Dr.Arvind Chavhan

Cite this article as:
Ghume Vaishnavi, Shingade Shilpa, Walhe Rajan. Study on biosurfactant production by beneficial lactic acid bacteria isolated from traditionally fermented Indian foods., Int. Res. Journal of Science & Engineering, 2021, Volume 9(1): 5-11.

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