RESEARCH ARTICLES

Volume 6 |Issue 6| Nov-Dec 2018                                 First published: 31 December 2018

Histochemical Study of Protein in Interstitial Cells in the Ovaries of bats Rousettus Leschenaulti, Megaderma Lyra Lyra, Hipposideros Speoris

Meshram Nitin P and Janbandhu KS

1Department of Zoology, S K Porwal College, Kamptee.(M.S). [INDIA]

2Govt. Institute of Science College, Nagpur.(M.S). [INDIA]

*Corresponding author Email: nitinmesh2015@gmail.com

Abstract

The presence of interstitial cells in the stromal region of the immature, inactive and active ovary of bat have been studied. The interstitial cells are larger than the stromal cells are polygonal in shape. Stroma and interstitial cells supplied with blood vessels. In the present study localization of protein was find out in the vicinity of interstitial cells. Histochemistry combines the methods of histology with those of biochemistry, to reveal the biochemical composition of tissues and cells. Protein serves variety of functions and acting as receptors, enzymes, conjugates, precursors and substrate for supplicating energy to the intricate events that occur in the ovary. For the localization of Protein, tissue (ovary) fixed in 10% formalin were used. The sections were cut at 4 – 6 µm. The presence of Protein demonstrated by mercuric bromophenol blue method. The hydrated sections were stained for 15 minutes and treated with 0.5 % aqueous acetic acid with 2 changes of 20 minutes. The section were dehydrated and cleaned in Xylene. The sections were treated with 100 ml Xylene and 0.5 ml n –butyl amine until they turned blue cleaned in Xylene and mounted in DPX. Protein stained blue. The distribution of mercuric bromophenol positive proteins in the interstitial cells in the ovaries of Rousettus leschenaulti, Megaderma lyra lyra and Hipposideros speoris bats observed in active as well as inactive condition. In inactive condition of ovary, protein staining in the interstitial cells showed moderate and in the active condition of ovary, protein staining showed high. Inactive ovary showed a positive activity of protein in interstitial cells and in most of the ovarian components. The cytoplasm of germinal epithelium cells and the stromal region was strongly stained. The primary, secondary, Graafian follicles and zona pellucida exhibited a strong stain. The corona radiata and theca folliculi showed a moderate reaction. The ovarian interstitial cells showed moderate to high reactions with mercury bromophenol blue in the pregnant ovary of all the three species of bats.

Keywords:Protein , Mercury Bromophenol blue , Interstitial Cells, Ovary

Editor: Dr.Arvind Chavhan

Cite this article as:
Meshram Nitin P and Janbandhu KS. Histochemical Study of Protein in Interstitial Cells in the Ovaries of bats Rousettus Leschenaulti, Megaderma Lyra Lyra, Hipposideros Speoris. Int. Res. Journal of Science & Engineering, 2018, 6 (6): 255-264.

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