RESEARCH ARTICLES

Volume 8 |Issue 4| July-August 2020                                 First published: 30 Augest 2020

Statistical Optimization of Cellulase production by haloalkaliphilic Bacillus sp. Isolated from Meteoritic Crater of Lonar Lake

Umrikar AM and Hamde VS

Department of Microbiology, Yogeshwari Mahavidyalaya, Ambajogai-431517, Dist. Beed (MS) India

Abstract

The enzyme cellulase has the ability to catalyze the carbohydrate which is a polymer of glucose linked with β-1, 4, glycosidic linkage. Such type of cellulase plays an important role in different industries like food, pharmaceutical, textile, leather, fuel etc. In the present investigation the potential haloalkaliphilic bacterial strains were used for the cellulase production. Because the present bacterial strain grows in two different extreme conditions such as high salt and high pH. The obtained cellulase after optimization may have some different chemical composition and improved activity as well. The six physicochemical parameters were used such as glucose, yeast extract, inoculums size, agitation, pH & temperature. Statistical model was designed on the basis of response surface methodology (RSM). In the obtained model among the 33 responses we got 7 th run combination showed the 1.2 U/mg of cellulase activity. When we find the analysis of variance (ANOVA) it reveals below 0.05 p values it indicated the proposed model was significant. The obtained p value was 0.0482 it means a significant data for the cellulase production by the Bacillus sp

Keywords:Lonar Lake, Soil sample, Haloalkaliphilic strains, cellulase, RSM.

Editor: Dr.Arvind Chavhan

Cite this article as:
Umrikar AM and Hamde VS. Statistical Optimization of Cellulase production by haloalkaliphilic Bacillus sp. Isolated from Meteoritic Crater of Lonar Lake, Int. Res. Journal of Science & Engineering, 2020, Volume 8(4): 125-130

Copyright:

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