RESEARCH ARTICLES

Volume 8 |Issue 4| July-August 2020                                 First published: 30 Augest 2020

Isolation and Screening of Cellulase Producing Bacterial Strain from Meteoritic Crater of Lonar Lake

Umrikar AM and Hamde VS

Department of Microbiology, Yogeshwari Mahavidyalaya, Ambajogai-431517, Dist. Beed (MS) India

Abstract

Lonar is the soda lake comprising biotechnologically important microbes with industrially important enzymes. The potential of the extremozymes is still underestimated. Lonar Lake has to be explored for its uniqueness, as it has the unique environmental condition with high salinity i. e. Halophilic condition and the high pH value i. e. alkaliphilic condition, the pH value reaches up to 10.5 and in such unique environment, cellulose is one of the largest organic components. In the present investigation, different samples from soil, water & sediment were collected for the isolation of potential cellulase producing bacteria. Among the collected samples, sediment microflora was isolated on CMC (Carboxy Methyl Cellulose) agar for the identification of cellulase producer. Total five isolates were screened on CMC agar medium among them LLI-1 (Lonar Lake Isolate) isolate showed maximum cellulolytic activity which was indicated by zone of clearance around the bacterial colony. The same bacterium was tentatively identified by culture dependent methods as Bacillus Sp. The potent Cellulase producer was enriched in CMC broth having pH 10.5 and incubated at 37°C for 24 hr.

Keywords:Bacillus Sp., CMC, Lonar lake, Isolates

Editor: Dr.Arvind Chavhan

Cite this article as:
Umrikar AM and Hamde VS. Isolation and Screening of Cellulase Producing Bacterial Strain from Meteoritic Crater of Lonar Lake, Int. Res. Journal of Science & Engineering, 2020, Volume 8(4): 141-144.

Copyright:

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/

References

1. Kanekar PP, Joshi AA, Kelkar AS, Borgave SB and Sarnaik SS. Alkaline Lonar Lake, India- A treasure of alkaliphilic and Halophilic bacteria, Proceedings of Taal2007: 12th world lake Conference, 2008, 1765-1774.

2. María De Lourdes Moreno, Dolores Pérez, María Teresa García And Encarnación Mellado. Halophilic Bacteria as a Source of Novel Hydrolytic Enzymes, Life, 2013, 3, 38-51.

3. Ashabil Aygan, Lutfiye Karcioglu and Burhan Arikan. Alkaline Thermostable and Halophilic Endoglucanasefrom Bacillus licheniformis C108, African Journal of Biotechnology, 2011, Vol. 10(5), pp. 789-796.

4. Jeong-Hwa C, Wan-Taek I, Qing-Mei L, Jae-Soo Y, Horikoshi K. Alkaliphiles: some applications of their products for biotechnology. Microbiol Mol Biol Rev.,1999, 63:735–750.

5. Siddarthan Venkatachalam, Manivannan Sivaprakash, Vasudevan Gowdaman And Solai Ramatchandirane Prabagaran. Bioprospecting Of Cellulase Producing Extremophilic Bacterial Isolates From India, British Microbiology Research Journal, 2014, 4(2): 138-150.

6. Bancy N. Muruga and Beatrice Anyango (2012), A Survey of Extremophilic Bacteria In Lake Magadi, Kenya, Columbia International Publishing American Journal Of Molecular And Cellular Biology , 2013, 1: 14-26.

7. Kshipra B. Deshmukh, Anupama P. Pathak, Mohan S. Karuppayil. Bacterial Diversity of Lonar Soda Lake Of India. Indian J Microbiol, 2011, 51(1):107–111.