RESEARCH ARTICLE

Volume 9 |Issue 1| January-February 2021                                 First published: 28 February 2021

Studies on extracellular protease production by Bacillus sp. Isolated from soil

Omprakash Kadam

Department of Microbiology, Digambarrao Bindu ACS College, Bhokar Dist. Nanded (MS)-431801 India

Email: omkadam2012@gmail.com

Abstract

The collected soil sample was serially diluted by serial dilution method and 10-5th tube was used for the isolation of protease producing bacterial strain. The zone of clearance was observed on SMA plate after 24hrs of incubation at 37°c temperature. The zone of clearance was recorded as 5 mm on SMA plate. The significant protease producing bacterial colony was used for biochemical analysis and it tentatively identified the bacterial strain as Bacillus strain. The various physico-chemical parameters were used to protease production such as incubation period, carbon, nitrogen source, temperature and pH. The incubation period for maximum production of protease at 24 hrs. It was 0.72 U/ml and minimum at 72 hrs. (0.40 U/ml). The maximum production of protease when fructose was used as carbon source (1.23 U/ml) and minimum at lactose and maltose that was 0.75 U/ml. The best nitrogen source for protease production was peptone (1.55 U/ml) and minimum at ammonium chloride (0.10 U/ml). The maximum production of protease was recorded in a medium at pH 8 that was 1.34 U/ml. The maximum biosynthesis of protease was recorded at 40°C that was 1.31 U/ml.

Keywords:Soil sample, Protease, physical and chemical components, Bacillus sp.

Editor: Dr.Arvind Chavhan

Cite this article as:
Kadam Omprakash. Studies on extracellular protease production by Bacillus sp. Isolated from soil, Int. Res. Journal of Science & Engineering, 2021, Volume 9(1): 21-27.

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