Nagarkar SS

Department of Botany, Adarsh Education Society’s Arts, Commerce and Science College, Hingoli 431513, MS, India.

E-mail: drssnagarkar@gmail.com

Abstract

Pollen grains contain number of metabolites which are responsible for physiological and metabolic activities. Pollen grains release the proteins, free amino acids and other chemical metabolites at different rates and quantities. The differences in these metabolites may be responsible for the control of rate and type of allergic reactions caused by pollen grains. Datura innoxia is a well-known pollen allergenic plant of India. Quantitative biochemical estimation in the form of carbohydrates, proteins, lipids, free amino acids, ash and moisture was done from the crude extract of the pollen of Datura innoxia L. During the present study, the pollen grains were found to be containing 1.28% of total reducing sugar, 3.28% of carbohydrates, 2.48% of nitrogen, 14.9% of crude protein, 0.69% of soluble protein, 0.86% of free amino acid, 1.23% of lipid, 8.77% of moisture and 2.68% of ash. The pollen grains were examined histochemically to assess qualitatively starch and lipid contents. The free amino acids and sugars were identified by thin layer chromatography.

Keywords:Datura innoxia, biochemical analysis, allergenic pollen.

Editor: Dr.Arvind Chavhan

Cite this article as:
Nagarkar SS. Biochemical Analysis of Pollen Grains of Datura innoxia L. for Screening its Allergenicity, Int. Res. Journal of Science & Engineering, 2021, Volume 9(4): 125-128.

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